$BOCBjDs6!(B

$B8!::K!>R2p(B

$B#R#N#A%j%"%k%?%$%`A}I}DjNL9q;:@hC<5;=Q!"#T#R#C$N0UL#$H0U5A(B
$B!=%N%m#T#R#C3+H/$H1~MQ!=(B


$B@nK\?R5A(B


$B4tIl8)J]7r4D6-8&5f=j(B
$B%N%m#T#R#C5;=QI>2A8&5fHID9(B
$B85!!8|@82J3XFCJL8&5fHID9(B


$B!Z=o8@![(B

$B!!#P#C#R$H#R#T!J5UE>o$K:$Fq$G$"$C$?!#(B

$B#T#R#C3+H/$O!"$=$N8f$7$,$?$$(BRNA$B$NJaB*8!=P$rM#0l2DG=$K$7$?@hC<5;=Q$@$H$$$($k!#!!Hs>o$KA!:Y$J%a%+%K%:%`$G5!G=$7!"#1%A%e!<%VDj29EY%j%"%/%7%g%s$GI8E*(BRNA$B$NFC0[E*A}I};:J*$r%b%K%?!<$G$-$k$N$@!#!!$o$?$7$O#R#N#AA}I}$N(BTRC$B3+H/%b%G%k$K$O!\:?(BRNA$B%&%$%k%9$G!V@$3&Cf$KB8:_$7?M0YE*@)8f$b]$H94$C$F$-$?!#!!%N%m(BTRC$B5;=Q3+H/$,5^B.$K?J$s$@$N$O!"$o$,8|@82J3X8&5fHI$dEv;~:_?&$N4tIl@8J*8&$G(B1999$BG/$^$G$K9qFb#6@iM>$N?)CfFG;v7o$d=8CD!&;6H/%&%$%k%9@-0_D21j$+$iBeI=#2#7#03t0J>e$N(BNV$B0dEA;R%G!<%?%Y!<%9$rJ]M-$7J;$;$F4tIl#G-5!"#G-63t#6e;T$5$lM[$NL\$r8+$k$3$H$H$J$C$?!#!!$=$l$K@hN)$DF1G/(B9$B7n$+$i$O!"NW>28!::3X$dIBM}4X783X2q!"46@w>I!&%&%$%k%93X2q$dF|JF0e3X2q5D%&%$%k%9@lLgIt2q$K5;=Q>R2p$H@.2L!&I>2A$rC`

$B!!$o$?$7$O$3$l$^$G$K!"A49q$NB?$/$N8&5fCg4V$K;Y$($i$l$F(BSRSV$B8&5f$N=l;~Be$N8&5fHI$+$i8|@82J3X8&5fHI$r7P$F%N%m(BTRC$B8&5fHI$X$H8&5fBP>]$,JQA+$7$?!#!!$3$l$i$OC;$$$o$,8&5f@836$NLsH>J,$,(BNV$B8&5f;K$K4X78$7$F$$$k!#!!$=$N4V!"A49q%&%$%k%9@-?)CfFGr9`F3F~$G8&5f$K$bBg57$,N)$A!"(BNV$BJ,;R1V3X$dM"F~5{2pN`$N=i%&%$%k%91x@wD4::!"2


$B!Z#T#R#C$K$h$k%N%m%&%$%k%98!=P![(B

$B!!(BTRC$B$OI8E*(Bm$B#R#N#A$KAjJd7k9g$9$kFC0[E*7V8wA}46(BINAF $B!J(BIntercalation Activating Fluorescence$B!K%W%m!<%V$H0lDj29EY$G(BRNA$B$rD>@\A}I}$9$k?WB.$G4J0W$J%j%"%k%?%$%`#R#N#A0dEA;R8!::$H$7$F3NN)$7%N%m%&%$%k%9(BRNA$B$N8!=P$O#G-5$H#G-68&5f;nLt$H$7$F8=:_!"!J3t!KEl%=!<$+$i;THNCf$G$"$k!#!!o$NH=Dj:GIQ;~4VCM$O(B30$BJ,DxEY$G!"?WB.$+$D9b46EY$G=>Mh$N#R#T!]#P#C#R$HF146EY$+$=$l0J>e$G$"$k!#(B



$B!Z0_D21j!&?)CfFG452A![(B

$B!!I>2A8!BN$NJ5JX(B252$B7o!J4doMQ$N%W%i%$%^!<%;%C%H!J4d

$B!Z7k2L![(B

$B!!(BRT-PCR$B$H(BTRC$B$N8!=PHf3S$G$O!"4dK!0lCWN($,(B89.2$B!s!JM[@-8!BN?t!'(BTRC 63$B7o!"(BRT-PCR 60$B7o!K!#?73c$H2,;3J5JX(B169$B7o$G$ON>K!0lCWN($,(B92.9$B!s!JM[@-8!BN?t!'(BTRC 108$B7o!"(BRT-PCR 104$B7o!K$G$"$C$?!#$^$?!"(BTRC$B$GM[@-8!BN$N(B97$B!s$O(B40$BJ,0JFb$KH=Dj2DG=%l%Y%k$K$"$C$?!#(B


$B!Z$^$H$a![(B

$B#1!K(B $B%N%m#T#R#C$O!"8!=P46EY$b(BRT-PCR$BK!$HF1Ey$+$=$l0J>e$G$"$j!"I8E*(BRNA$B$rD>@\A}I}$9$k$?$a(BRT$BH?1~$,ITMW$G!"H=Dj$^$G$N=jMW;~4V$O(B60$BJ,0JFb$H?WB.!#(B
$B#2!K(B $B%N%m#T#R#C$OA}I}H?1~8e$NEE5$1KF0$d(BDNA$B%O%$%V%j%@%$% $B#3!K(B $B%N%m#T#R#C$O!"?WB.H=Dj$,5a$a$i$l$kNW>2!"=8CD0_D21j$d?)CfFG;v7o860x5fL@!J45e2

$B!Z;29M@=IJ$N8!:w(BWeb$B%5%$%H![(B

$BEl%=!<3t<02q URL$B!!(Bhttp://www.tosoh.co.jp/science/trc/reagent.html
$B0dEA;R8!::%7%9%F%`!"%j%"%k%?%$%`7V8w%b%K%?!

Rapid and Easy Detection of Norovirus RNA by Using Real-Time TRC1 System of New NAT2

Hiroyoshi Kawamoto1, Tsuyoshi Kuzuguchi2, Noriyoshi Masuda3, Kiyoshi Yasukawa3, Toshinori Hayashi3, Koichi Saito4, Makoto Nishikawa5 and Ritushi Fujii6

1 Gifu Pref. Inst. Health and Environmental Sciences, 2 Gifu Pref. Inst. Bio-industrial Technology,
3 Scientific Instruments Division, TOSOH Corporation, 4 Iwate Pref., 5 Niigata Pref., and 6 Okayama Pref. Inst. Public Health

TRC1, Transcription-Reverse transcription Concerted reaction, NAT2, Nucleic acid Amplification Test, $B!!(BKey word; NAT, TRC, Norovirus, RT-PCR, RNA amplification, Real-time amplification, TRC monitor, RNA extraction kit


$B!Ze$2!"K\8&5f2q0w3F0L$N99$J$k$4H/E8$H$47r>!$r5'$j$^$9!#(B


$B"+(BBack$B!!!!!!(B$B>6O?L\$B!!!!!!(BNext$B"*(B
$B%H%C%W%Z!<%8$X$b$I$k(B