Significance
of
Chinese
Medicine-TSJN
in
Insulin
Resistance,
Hypertension
and
Diabetes
Mellitus
Li
Yi,
Li
Wenrui
(Department
of
RUI
DONG
Diabetes
Center
of
TCM
and
WM,Beijing
Hospital,
China)
N.
Ura,
K.
Shimamoto
(Second
Department
of
Internal
Medicine
School
of
Medicine,
Sapporo
Medical
University,
Japan)
TSJN
is
produced
by
traditional
Chinese
medicines,
Ginsehg,
Milkvetch
Root,
Rhubarb,
Golden
Thread
Rhizome,
Leech,
Umbellatus
Pore-fungus.
Before
the
next
study,
we
selected
21
patients
with
type
2
DM
according
to
Morgenson's
standard
of
the
early
stage
diabetic
nephropathy,
and
randomly
divided
into
two
groups.
One
group
was
collators
(n=7);
the
other
one
was
treatment
group
with
TSJN
(n=14;
6g/day)
for
6
months.
Urinary
albumin
excretion
rate
(UAER)
on
24-hours
significantly
decreased
in
12
patients
of
treatment
group,
while
only
1
patients
decreased
UAER
in
collators
group.
Thus
TSJN
has
the
effect
of
renal
protection
in
diabetic
nephropathy.
The
next
we
investigated
the
effects
of
TSJN
on
insulin
resistance
and
hypertension
in
fructose-fed
rats
(FFR).
Six-week-old
male
Sprague-Dawley
rats
were
fed
either
normal
rat
chow
(control;
n=20)
or
a
fructose-rich
chow
(FFR)
for
6
weeks.
FFR
was
divided
into
FFR＋vehicle
(FFR;
n=18,
2.5%
gum
arabic
solution
by
gavage),
FFR＋2
weeks
treatment
of
TSJN
(FFR＋TS-2;
n=21,
800mg/kg/day
of
TSJN),
and
FFR＋4
weeks
treatment
of
TSJN
(FFR＋TS-4;
n=11).
Systolic
blood
pressure
(SBP)
was
measured
by
tail
cuff
method.
At
the
end
of
the
protocol,
the
euglycemic
hyperinsulinemic
glucose
clamp
technique
was
performed
to
estimate
insulin
sensitivity
(M
value),
and
then
the
soleus
muscle
was
dissected
out
for
determination
of
muscle
fiber
composition
by
ATPase
method.
M
value
in
FFR
(10.9±0.6mg/kg/min)
was
significantly
lower
than
in
control
(15.4±0.4),
while
TSJN
significantly
improved
the
M
value
(FFR＋TS-2;
15.1±0.5,
FFR＋TS-4;
14.5±0.5)
in
FFR.
Although
the
composite
ratio
of
typeⅠ
fibers
in
the
soleus
muscle
in
FFR
(75.0±1.7%)
was
significantly
lower
than
in
control
(81.7±1.5),
that
in
FFR＋TS-2
was
significantly
higher
than
in
FFR
and
not
differ
to
that
in
control.
SBP
in
FFR
(155±3nnHg)
was
significantly
higher
than
in
control
(142±2).
Although
SBP
in
FFR＋TS-2
(155±2)
was
not
differ
to
that
in
FFR,
SBP
in
FFR＋TS-4(137±3)
was
significantly
lower
than
in
FFR.
These
results
suggest
that
1)
one
of
the
mechanisms
of
insulin
resistance
and
hypertension
may
be
the
change
of
muscle
fiber
composition,
2)
TSJN
may
improve
insulin
resistance
by
modulation
of
muscle
fiber
composition,
and
3)
long
term
treatment
of
TSJN
may
improve
hypertension
by
improving
insulin
resistance
and
compensately
hyperinsulinemia
in
insulin
resistant
hypertensive
FFR.
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